Isolate and Sub-fractionate Cell Membranes from Caulobacter crescentus.
نویسنده
چکیده
Cell membranes from Caulobacter can be isolated and separated into inner and outer membranes according their characteristic buoyant densities on a sucrose gradient. Fractionation can be used to determine the localisation of uncharacterised proteins and to enrich protein complexes present in either of these membranes for biochemical analysis such blue-native PAGE and immunoprecipitation.
منابع مشابه
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ورودعنوان ژورنال:
- Bio-protocol
دوره 2 20 شماره
صفحات -
تاریخ انتشار 2012